Adsorption Density Control of Ferritin Molecules by Multistep Alternate Coating

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概要

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• We have developed a process to control adsorption density of ferritin molecules on SiO2 surface in the high-density regime. We use two types of ferritin molecules: titanium-binding peptide ferritin (TBF) and Fer8S. The former has a property to get adsorbed on the SiO2 surface, and it is positively charged in the buffer solution. The latter is negatively charged in the solution. Exposure of the TBF solution on the substrate followed by rinse in water and drying leads to 4.6\times 10^{3} molecules/μm2 of adsorption density, corresponding to a half of the coverage of the whole surface. Subsequent drop of the Fer8S solution leads to 6.0\times 10^{3} molecules/μm2, and repeating this alternate coating process enables the full coverage of the surface. We also discuss the dominant factors that determine the adsorption patterns using Monte Carlo simulations.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2011-06-25

著者

• Yamashita Ichiro

  Graduate School Of Materials Science Nara Institute Of Science And Technology

• Isono Yoshitada

  Department Of Engineering Ritsumeikan University

• Zheng Bin

  Graduate School Of Materials Science Nara Institute Of Science And Technology

• Uraoka Yukiharu

  Graduate School of Material Science, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara 630-0192, Japan

• Isono Yoshitada

  Department of Mechanical Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan

• Hanasaki Itsuo

  Department of Mechanical Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan

• Zheng Bin

  Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan

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