Lipid Nanotube Encapsulating Method in Low-Energy Scanning Transmission Electron Microscopy Analyses

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概要

• 論文の詳細を見る

• The lipid nanotube (LNT) encapsulating method is a rational sample fixation method that can be used to mount samples for transmission electron microscopy analyses. By employing the LNT encapsulating method in 30 kV low-voltage scanning transmission electron microscopy (LV-STEM), it is possible to record multiangle images of ferritin without using the negative staining method. We have also recorded a tilted series of high-contrast LV-STEM images and reconstructed three-dimensional images. These results show that LNTs have sufficient durability for LV electron beam, and indicate the potential of the LNT encapsulating method as a sample fixation method of LV electron microscopy.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2009-09-25

著者

• Yamashita Ichiro

  Graduate School Of Materials Science Nara Institute Of Science And Technology

• Yamane Midori

  Graduate School Of Frontier Biosciences Osaka University

• Furusho Hirotoshi

  Research Center For Ultra-high Voltage Electron Microscopy Osaka University

• Mori Hirotaro

  Research Center For Ultra-high Voltage Electron Microscopy Osaka University

• Takaoka Akio

  Research Center For Uhvem Osaka Univ.

• Mori Hirotaro

  Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, 7-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

• Takaoka Akio

  Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, 7-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

• Masuda Mitsutoshi

  Solution-Oriented Research for Science and Technology (SORST), Japan Science and Technology Agency (JST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

• Shimizu Toshimi

  Solution-Oriented Research for Science and Technology (SORST), Japan Science and Technology Agency (JST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

• Mishima Yumiko

  Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Rue Charles Sadron F45071, Orléans, France

• Kameta Naohiro

  Solution-Oriented Research for Science and Technology (SORST), Japan Science and Technology Agency (JST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

• Asakawa Masumi

  Solution-Oriented Research for Science and Technology (SORST), Japan Science and Technology Agency (JST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

• Mishima Yumiko

  Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Rue Charles Sadron F45071, Orléans, France

• Yamane Midori

  Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-0192, Japan

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