Lipid Nanotube Encapsulating Method for Two- and Three-Dimensional Transmission Electron Microscopy Analyses of Cage-Shaped Proteins

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概要

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• A novel, sample-fixation technique for transmission electron microscopy (TEM) is proposed. We encapsulated various samples in one-dimensional hollow cylinders of a glycolipid nanotube (LNT) and found it to be highly stable, flexible, and reproducible. The cylindrical shape of the LNT allows us to observe nanomaterials consistently even when the samples are rotated around the tube axis. This is critical in improving TEM computed tomography (CT), which suffers from the loss of the electron beam at high tilting angles because of the increased effective thickness of the supporting film. This technique makes it possible to reconstruct three-dimensional images from weak-signal TEM images. In this work, we examined two types of cage-shaped proteins, ferritin, and DNA-binding protein from starved cells (Dps). We reconstructed three-dimensional images of Fe cores from zero-loss images or from electron energy-loss spectroscopy (EELS) mapping images obtained with the Fe M2,3 edge. We also applied negative staining and vitreous ice embedding (VIE). These results show the potential of the LNT encapsulation technique.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2008-01-25

著者

• Yamashita Ichiro

  Graduate School Of Materials Science Nara Institute Of Science And Technology

• Mishima Yumiko

  Crest Japan Science And Technology Agency

• SHIMIZU Toshimi

  SORST, Japan Science and Technology Agency

• Furusho Hirotoshi

  Research Center For Ultra-high Voltage Electron Microscopy Osaka University

• Masuda Mitsutoshi

  Sorst Japan Science And Technology Agency

• Masuda Mitsutoshi

  SORST, Japan Science and Technology Agency (JST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

• Kameta Norihiro

  SORST, Japan Science and Technology Agency (JST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

• Furusho Hirotoshi

  Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, 7-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

• Mishima Yumiko

  CREST, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

関連論文

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• A New Silicon Quantum-Well Structure with Controlled Diameter and Thickness Fabricated with Ferritin Iron Core Mask and Chlorine Neutral Beam Etching
• Reduction of a Ferritin Core Embedded in Silicon Oxide Film for An Application to Floating Gate Memory
• 1P-307 球殻上タンパク質アポフェリチンを用いた化合物半導体ZnSナノ粒子の作製(バイオエンジニアリング,第46回日本生物物理学会年会)
• Effect of N-terminal Residues on the Structural Stability of Recombinant Horse L-chain Apoferritin in an Acidic Environment
• Fabrication of Gold Sulfide Nanoparticles Using the Protein Cage of Apoferritin
• Electrostatic Placement of Nanodots onto Silicon Substrate Using Ferritin Protein Supramolecules with Control of Electrostatic Interaction in Solution
• Floating Nanodot Gate Memory Devices Based on Biomineralized Inorganic Nanodot Array as a Storage Node
• Adsorption Properties of a Gold-Binding Peptide Assessed by its Attachment to a Recombinant Apoferritin Molecule
• Floating Gate MOS Capacitor with High-Density Nanodots Array Produced by Protein Supramolecule
• Enhancement of crystal growth rate of Bio-Nano Crystallization by Pulsed Rapid Thermal Annealing
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• Effective Shortening in Length of Glycolipid Nanotubes with High Axial Ratios
• Location and Density Control of Carbon Nanotubes Synthesized Using Ferritin Molecules
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• Plasmon Absorbance of SiO
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