Three-dimensional, Computer-tomographic Analysis of Membrane Proteins (TrkA, caveolin, clathrin) in PC12 Cells
スポンサーリンク
概要
- 論文の詳細を見る
Signaling of nerve growth factor (NGF) and its receptor (TrkA) promotes neuronal differentiation, synapse formation and survival. It has been known that the complex of NGF and TrkA is internalized into the cytoplasm and transported for further signal transduction, but the ultrastructural information of this process is virtually unknown. In order to clarify the relation-ship between the internalization of TrkA and the membrane-associated proteins (caveolin and clathrin), the localization and three-dimensional structures of those proteins were examined with computer tomography of high voltage electron microscopy in PC12 cells. TrkA immunoreactivity was found only at definite areas in the plasma membrane, as ring and cluster structures. Its 3D image indicated that those cluster structures contained small pits, which did not appear to be typical caveolae in size and shape. 3D images of clathrin and caveolin-1 immunoreactivities indicated that the formation of those small pits was associated with clathrin, but not with caveolin-1. Caveolin-1 immunoreactivity was found as a mesh-like structure just beneath the plasma membrane. These results suggest that clathrin rather than caveolin is mainly involved in the process of TrkA internalization, at least in differentiated PC12 cells.
- 日本組織細胞化学会の論文
著者
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吉村 亮一
国立循環器病センター研究所循環動態機能部
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吉村 亮一
京都工繊大・応用生物
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Endo Yasuhisa
Division Of Applied Biology Kyoto Institute Of Technology
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Takaoka Akio
Research Center For Ultra-high Voltage Electron Microscopy Osaka University
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Yoshimura Ryoichi
Division Of Applied Biology Kyoto Institute Of Technology Graduate School Of Science And Technology
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Yoshimura Ryoichi
Department Of Applied Biology Kyoto Institute Of Technology
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Yoshimura Ryoichi
Department Of Cardiovascula Dynamics National Cardiovascular Center Research Institute
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Yoshimura Ryoichi
Dept. Applied Biol Kyoto Inst. Tech.
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Yoshimura Ryoichi
Department Of Neuroanatomy Bio Edical Research Center Osaka University Medical School
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有井 達夫
National Institute For Physiologinal Sciences
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Takaoka Akio
Research Center For Uhvem Osaka Univ.
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Nishida Tomoki
Division of Applied Biology, Kyoto Institute of Technology
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Arii Tatsuo
Centerfor Brain Experiment, National Institute for Physiological Sciences
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Arii Tatuo
Center For Brain Experiment National Institute For Physiological Sciences
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Nishida Tomoki
Division Of Applied Biology Kyoto Institute Of Technology
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Nisida Tomoki
Department Of Applied Biology Kyoto Institute Of Technology
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Takaoka Akio
Research Center for Ultra-High Voltage Electron Microscopy, Osaka University
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