Physicochemical Characteristics and Antitumor Activities of a Highly Branched Fungal (1→3)-β-D-Glucan, OL-2,Isolated from Omphalia lapidescens

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Physicochemical properties and antitumor activities of a fungal (1→3)-β-D-glucan, OL-2,isolated from Leiwan (Omphalia lapidescens) were examined. OL-2 showed sharp signals on carbon-13 nuclear magnetic resonance spectrum in dimethylsulfoxide-d_6 as a solvent, and these signals were significantly reduced by the addition of distilled water to the concentration of 20%. This phenomenon is consistent with the general property of the gel forming (1→3)-β-D-glucan. Binding of OL-2 to Congo red induced a significant change of λ_<max> to a longer wavelength, and the concentration to induce gel to sol transition was about 0.7 N; in contrast, the concentration was about 0.2 N in the cases of SPG and curdlan. These observations suggested that the gel structure would be significantly stabilized in the case of OL-2. OL-2 showed no or low antitumor activity against the solid form of Sarcoma 180 by intraperitoneal and intralesional administrations; however, it was effective on the ascites form of Sarcoma 180. Of interest, OL-2 also showed significant antitumor activity against the ascites form of MH-134 when administered with 5-fluorouracil. These results indicated that OL-2 showed characteristic features regarding its physicochemical properties and antitumor activity.
社団法人日本薬学会の論文
1992-08-25