Conformation of Grifolan in the Fruit Body of Grifola frondosa Assessed by Carbon-13 Cross Polarization-Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy(Biological)

概要

論文の詳細を見る
Grifolafrondosa produces an antitumor(1→3)-β-D-glucan, grifolan, which possesses two kinds of conformations in the solid state, i.e., helix (curdlan type) and native (laminaran type)(Ohno et a/., Chem. Pharm. Bull, 34, 2555(1986)), In this paper, the glucan conformation in the fruit body was examined by using carbon-13 cross polarization-magic angle spinning(CP/MAS) nuclear magnetic resonance(NMR) spectroscopy. Before analysis, the fruit body was treated as follows: amylase digestion, amylase digestion-periodate-borohydride treatment, hot water extraction, cold alkali extraction, or hot alkali extraction. Except for the cold and the hot alkali-extracted fruit bodies, all preparations showed C-3 signals at about 86ppm, resembling those of the native form. These findings suggested that the (1→3)-β-D-glucan conformation in the fruit body is the "native form."
社団法人日本薬学会の論文
1987-06-25