Isolation of Mitogenic Substance from Sclerotia of Sclerotinia sclerotiorum IFO 9395 Extracted with Phosphate Buffer
スポンサーリンク
概要
- 論文の詳細を見る
The buffer extracts (3S) of sclerotia of Sclerotinia sclerotiorum IFO 9395 contained mitogenic substance(s) to murine splenocytes (Shinohara et al. Chem. Pharm. Bull., 38,2219 (1990)). Although the native 3S was slightly mitogenic, heating of 3S induced significant mitogenic activity. To isolate the mitogen, we separated 3S by ion-exchange and gel filtration chromatographies. The isolated mitogen, named sclerogen, has a molecular mass of 32 kilodaltons (kDa) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the isoelectric point (pI) of 5.9 by chromatofocusing. Sclerogen was significantly mitogenic in vitro against murine splenocytes after heat denaturation, and also showed the augmentation of the primary mixed lymphocyte reaction (MLR) in vitro. However, sclerogen did not show the activation of an alternative pathway of complement and hemagglutination activity. These results suggest that sclerogen is a unique mitogen which differs from lectins and shows mitogenicity after heat denaturation.
- 社団法人日本薬学会の論文
- 1991-05-25
著者
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大野 尚仁
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
東京薬科大学 薬 免疫
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宿前 利郎
東京薬科大学
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大野 尚仁
東京薬科大学免疫学
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大野 尚仁
帝京大学 医学部微生物学講座
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篠原 久実
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy
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篠原 久実
Tokyo College of Pharmacy
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Ohno N
Lab. Immunopharmacolgy Of Microbial Products Tokyo University Of Pharmacy And Life Science School Of
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篠原 久実
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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