Structural Analysis of Alkaline-Soluble Polysaccharide, P-1,from the Kernels of Prunus mume SIEB. et ZUCC.
スポンサーリンク
概要
- 論文の詳細を見る
A polysaccharide fraction extracted with cold 0.5M NaOH from the kernels of Prunus mume exhlbited some biological activities. A polysaccharide, P-1,was purified from the 0.5M NaOH extract by ion-exchange chromatography and gel-flltration. The results of the structural analysis of P-1 to determine the relationship between the activities and the structure are described in this paper. In the mild acid hydrolysis of P-1,the nondialyzable hydrolysate (I-3) believed to be its core portion was obtained. The yield of I-3 was 26.0% and contained 59.8% uronic acid as galacturonic acid (GalA). The neutral sugars of I-3 were composed of rhamnose, xylose and galactose in a molar ratio of 1.0 : 3.4 : 0.3 following analysis by gas-liquid chromatography. The molecular weight of I-3 was estimated to be ca. 14000 by gel-filtration on Toyopearl HW55F. I-3 exhibited the mitogenic activity toward spleen cells as well as P-1. These facts appeared to confirm that I-3 was the core part of P-1 and important for its biological activity. I-3 was successfully reduced by the Taylor and Conrad method to avoid so much repetition. Methylation analysis of the reduced hydrolysate by gas-liquid chromatography and gas chromatography-mass spectroscopy showed that the ratio of 1,4-linked galactopyranosyl and 1,3,4-linked galactopyranosyl residues were significantly increased in comparison with native I-3. These results suggested that I-3 was composed of 1,4- and 1,3,4-linked galacturonic acid residues in the main chain.
- 社団法人日本薬学会の論文
- 1995-03-15
著者
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大野 尚仁
東京薬科大学薬学部免疫学教室
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大野 尚仁
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
東京薬科大学 薬 免疫
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宿前 利郎
東京薬科大学
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堂ケ崎 知格
Faculty of Environmental Health, Azabu University
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村上 一
Faculty of Environmental Health, Azabu University
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西島 基弘
Tokyo Metropolitan Research Laboratory of Public Health
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宮崎 利夫
Tokyo College of Pharmacy
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大野 尚仁
東京薬大免疫
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堂ヶ崎 知格
麻布大学
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大野 尚仁
東京薬科大学免疫学
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大野 尚仁
帝京大学 医学部微生物学講座
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大野 尚仁
東京薬科大学薬学部第一微生物学教室
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村上 一
Faculty Of Environmental Health Azabu University
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堂ヶ崎 知格
東京都立衛生研究所
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Ohno N
Lab. Immunopharmacolgy Of Microbial Products Tokyo University Of Pharmacy And Life Science School Of
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宮崎 利夫
Tokyo University Of Pharmacy And Life Science (formerly Tokyo College Of Pharmacy)
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宮崎 利夫
東京薬科大学 薬
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三浦 敏秀
Tokyo University Of Pharmacy And Life Science (formerly Tokyo College Of Pharmacy) Kitasato Universi
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堂ケ崎 知格
麻布大
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