Separation of the Hot Water Extracts of Sclerotia of Sclerotinia sclerotiorum IFO 9395 into Mitogenic and Antitumor-Active Subfractions
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概要
- 論文の詳細を見る
The hot water extract of sclerotia of Sclerotinia sclerotiorum IFO 9395 (TSHW) was divided into representative fractions by ammonium sulfate and ethanol precipitations, and (1→3)-β-D-glucanase treatment. The ammonium sulfate and ethanol precipitations gave a (1→3)-β-D-glucan fraction (TSG) and a mannan fraction (TSM). After the degradation of (1→3)-β-D-glucan in TSHW by (1→3)-β-D-glucanase treatment, a water-isoluble protein fraction (EDP) and supernatant (EDS) were obtained. Among these fractions, the mitogenic and antitumor activities were mainly observed in EDP and TSG, respectivety. On the other hand, the stimulatory effect on the reticuloendothelial system was mainly found in EDP and EDS, and a weak effect was observed in TSG. These findings suggest that the mitogenic and antitumor activities of TSHW were mainly due to the protein and (1→3)-β-D-glucan, respectively, and that the mitogenic substance (EDP) is tightly bound to (1→3)-β-D-glucan (TSG) in TSHW, accounting for its solubility in aqueous solution.
- 社団法人日本薬学会の論文
- 1989-08-25
著者
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大野 尚仁
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
東京薬科大学 薬 免疫
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宿前 利郎
東京薬科大学
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大野 尚仁
東京薬科大学免疫学
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大野 尚仁
帝京大学 医学部微生物学講座
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篠原 久実
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy
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篠原 久実
Tokyo College of Pharmacy
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Ohno N
Lab. Immunopharmacolgy Of Microbial Products Tokyo University Of Pharmacy And Life Science School Of
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篠原 久実
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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