Rapid Measurement of Phagocytosis by Macrophages
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概要
- 論文の詳細を見る
A rapid method for measurement of phagocytosis by macrophages was investigated by the use of fluorescein-conjugated zymosan (F1-zymosan) particles. Adherent peritoneal cells obtained from mice were used as macrophages. The fluorecence intensity of F1-zymosan measured with a fluorescence spectrophotometer was proportional to the number of particles. Fl-zymosan particles were incubated with macrophages in a flat-bottomed 96-well tissue culture plate at 37℃ in a CO_2-incubator. After the removal of nonphagocytized particles, the fluoreascnece intensity of particles phagocytized by macrophages was measured with a fluorescence spectrophotometer (microplate reader). In the time course assay, the fluorescence intensity reached a maximum 20 min after the initiation of incubation, and thereafter maintained a constant level till 1 h in both normal and OK-432 injected groups. It was shown that the fluorescence intensity was dependent on the numbers of macrophages and Fl-zymosan particles in both normal and OK-432-injected groups when different numbers of particles (1×10^6,5×10^6 or 1×10^7) were added to cultures each containing different numbers of macrophages (1×10^5,2×10^5 or 4×10^5). The macrophages obtained from mice which were injected intraperitoneally (i.p.) with Propionibacterium acnes or proteose peptone as well as OK-432 showed higher phagocytic activity. Furthermore, it was also shown that the phagocytic activity measured with a fluorescence spectophotometer agreed with that measured with a fluorescence microscope. The Fl-zymosan particles were applicable to the phagocytosis assay of opsonized particles.These results indicated that the method described here is useful for determination of macrophage phagocytic activity.
- 社団法人日本薬学会の論文
- 1988-12-25
著者
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安達 禎之
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Scienc
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宿前 利郎
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Scienc
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宿前 利郎
東京薬科大学 薬 免疫
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宿前 利郎
東京薬科大学
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鈴木 巌
Tokyo College of Pharmacy
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鈴木 巖
Tokyo College of Pharmacy
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鈴木 巌
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy
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田中 秀樹
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy
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安達 禎之
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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安達 禎之
東京薬科大学
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田中 秀樹
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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安達 禎之
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy
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