Monoclonal Antibody to Proteoglycan Derived from Grifola frondosa (Maitake)
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概要
- 論文の詳細を見る
A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass ; Ig G_<2b>), designated MPG2,reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed that MAb MPG2 reacted with lysosomal proteoglycan and (1→6)-β-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1→3)-β-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.
- 社団法人日本薬学会の論文
- 1994-04-15
著者
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安達 禎之
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Scienc
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平田 昭夫
Research Laboratory, Taito Co., Ltd. Kobe-Gakuin University
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伊藤 渡
Research Laboratory, Taito Co., Ltd. Kobe-Gakuin University
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平田 昭夫
Research Laboratory Taito Co. Ltd.
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菰田 真紀子
Research Laboratory, Taito Co., Ltd.
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田畑 [けん]吾
Research Laboratory, Taito Co., Ltd.
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菅原 勇
Dept. of Pathology, Saitama Medical Center, Saitama Medical School
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伊藤 渡
Research Laboratory Taito Co. Ltd.
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安達 禎之
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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菅原 勇
Dept. Of Pathology Saitama Medical Center Saitama Medical School
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田畑 吾
Research Laboratory Taito Co. Ltd.
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菰田 真紀子
Research Laboratory Taito Co. Ltd.
関連論文
- Modulation of the Antitumor Effect and Tissue Distribution of Highly Branched (1→3)-β-D-Glucan, SSG, by Carrageenan
- Relationship between the Tissue Distribution and Antitumor Activity of Highly Branched (1→3)-β-D-Glucan, SSG
- The Inhibitory Effect of Sulfated Schizophyllans and Related Oligosaccharides on Coagulation and Fibrinolysis
- Structure-Activity Relationship of (1→3)-β-D-Glucans in the Induction of Cytokine Production from Macrophages, in Vitro
- Comparison of the Immunopharmacological Activities of Triple and Single-Helical Schizophyllan in Mice
- Comparison of the Blood Clearance of Triple- and Single-Helical Schizophyllan in Mice
- Enhancement of Cytokine Production by Macrophages Stimulated with (1→3)-β-D-Glucan, Grifolan (GRN), Isolated from Grifola frondosa
- Preparation and Antigen Specificity of an Anti-(1→3)-β-D-glucan Antibody
- Analysis of Cytokine mRNAs Induced by the Administration of a Highly Branched (1→3)-β-D-Glucan, OL-2
- Expression of Interleukin 1 Family mRNAs by a Highly Branched (1→3)-β-D-Glucan, OL-2
- Application of Limulus Test (G Pathway) for the Detection of Different Conformers of (1→3)-β-D-Glucans
- Inhibitory Effect of β-Glucans on Zymosan-Mediated Hydrogen Peroxide Production by Murine Peritoneal Macrophages in Vitro
- Binding of Lysozyme to Synthetic Monosaccharide Lipid A Analogue, GLA60
- Characterization of Mitogenic Substances in the Hot Water Extracts of Bupleuri Radix
- An Improved Sandwich ELISA Method for the Determination of Immunoreactive Schizophyllan (SPG)
- Monoclonal Antibody to Proteoglycan Derived from Grifola frondosa (Maitake)
- Detection of Immunoreactive Schizophyllan by Solid-Phase Enzyme-Linked Immunosorbent Assay
- 138 シゾフィラン : 構造と免疫調節作用
- Change of Biological Activities of (1→3)-β-D-Glucan from Grifola frondosa upon Molecular Weight Reduction by Heat Treatment
- Rapid Measurement of Phagocytosis by Macrophages
- Conformational Changes of the Two Different Conformers of Grifolan in Sodium Hydroxide, Urea or Dimethylsulfoxide Solution(Biological)