Binding of Lysozyme to Synthetic Monosaccharide Lipid A Analogue, GLA60
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概要
- 論文の詳細を見る
Recent studies by our group suggested that lysozyme (LZM) has a high affinity for bacterial lipopolysaccharide (LPS) of both the smooth and rough forms, and inhibits various immunomodulatory activities of LPS. GLA60 is a synthetic monosaccharide analogue of bacterial lipid A, well-know as sharing large part of lipid A activities but with very low toxicity. In this study, we characterized the interaction of LZM with GLA60 in comparison to that with E. coli 0111 LPS (smooth form), taking a physicochemical approach. Using a dansylated lysozyme probe (DNS-LZM), LZM was found to bind to GLA60 in all 3 of its forms, free acid, triethylamine (TEA) salt and bovine serum albumin (BSA) complex of GLA60,as well as natural LPS. Compared with LPS, the complex formation of the TEA salt was weakly dependent on temperature and incubation time. LZM also bound to biologically inactive GLA analogues, GLA64 and GLA69,at a high affinity, as well as to GLA 60. By using chemically modified LZM, it was found that the ionic as well as hydrophobic interactions are important for the complex formation.
- 社団法人日本薬学会の論文
- 1993-03-15
著者
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大野 尚仁
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Scienc
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安達 禎之
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Scienc
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宿前 利郎
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Scienc
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宿前 利郎
東京薬科大学 薬 免疫
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宿前 利郎
東京薬科大学
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谷田 宣文
Department of Microbiology, Jichi Medical School
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松浦 基博
Department of Microbiology, Jichi Medical School
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中野 昌康
Department of Microbiology, Jichi Medical School
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木曽 真
Department of Applied Bioorganic Chemistry, Gifu University
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長谷川 明
Department of Applied Bioorganic Chemistry, Gifu University
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安達 禎之
東京薬科大学免疫学
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長谷川 明
岐阜大学農学部
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木曽 真
岐阜大学農学部
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木曽 真
岐阜大 農
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大野 尚仁
東京薬科大学免疫学
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大野 尚仁
帝京大学 医学部微生物学講座
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木曽 真
岐阜大学
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安達 禎之
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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長谷川 明
日本大学薬学部
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Ohno N
Lab. Immunopharmacolgy Of Microbial Products Tokyo University Of Pharmacy And Life Science School Of
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Adachi Yoshiyuki
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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松浦 基博
自治医大・微生物
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松浦 基博
自治医科大学・微生物学教室
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谷田 宣文
Department Of Microbiology Jichi Medical School
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木曽 真
Department Of Applied Bio-organic Chemistry Gifu University
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