Heat Induced Generation of the Mitogenic Substance(s) Responding to Murine Splenocytes Obtained from Sclerotia of Sclerotinia sclerotiorum IFO 9395
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概要
- 論文の詳細を見る
We have demonstrated that hot water extracts of sclerotia of Sclerotinia sclerotiorum IFO 9395 (TSHW) show various immunomodulating activities and mitogenic substance(s) were recovered from the β-1,3-glucanase resistant-fraction (EDF) (Shinohara et al. Chem. Pharm. Bull., 37,2174 (1989)). In this paper, we examined whether or not the mitogenic substance(s) were also obtained from the other methods, phosphate buffer extraction. Although the native extracts (3S-M) sterilized with a membranse filter showed a slight mitogenicity to murine splenocytes, 3S-M denatured in boiling water (3S-MB) showed significant activity. Treatment of 3S-M for only 1 min in boiling water or 10 min at 70 ℃ was sufficient to show significant mitogenic activity. After heat treatment of 3S-M in boiling water for 30 s, the main band corresponding to that of 3S-M was not clearly observed. Instead, new bands appeared at the top of the gel in normal-polyacrylamide gel electrophoresis (normal-PAGE), suggesting that many physicochemical changes occurred during that heat treatment. These findings suggest that heat denaturation of substance(s) from sclerotia was one of the triggering mechanisms expressing mitogenic activity to murine splenocytes.
- 社団法人日本薬学会の論文
- 1990-08-25
著者
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大野 尚仁
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
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宿前 利郎
東京薬科大学 薬 免疫
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宿前 利郎
東京薬科大学
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大野 尚仁
東京薬科大学免疫学
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大野 尚仁
帝京大学 医学部微生物学講座
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篠原 久実
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy
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篠原 久実
Tokyo College of Pharmacy
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Ohno N
Lab. Immunopharmacolgy Of Microbial Products Tokyo University Of Pharmacy And Life Science School Of
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篠原 久実
Laboratory Of Immunopharmacology Of Microbial Products Tokyo College Of Pharmacy
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