Characterization of the Antitumor Clucan Obtained from Liquid-Cultured Grifola frondosa
スポンサーリンク
概要
- 論文の詳細を見る
Chemical characterization of the extracts, and purification and structural characterization of an antitumor glucan obtained from liquid-cultured mycelium of Grifola frondosa are described in this paper. The mycelium (13 g dry weight/1 culture) was extracted successively with hot water (LMHW, 14.9 g/100 g mycelium), cold alkali (LMCA, 6.3 g/100 g mycelium), and hot alkali (LMHA, 4.5 g/100 g mycelium). Each extract was dialyzed and then the polysaccharide fraction was precipitated with ethanol. Another portion of the mycelium was incubated with a buffer composed of glucose (5.0%) and citric acid, pH 4.5,for 3 d and the supernatant was obtained (LELFD, 50 g/100 g mycelium). The brothe was dialyzed and the nondialyzable fraction was precipitated with ethanol (LLFD, 1.3 g/1 culture). All of the above fractions were composed of glucose as the major carbohydrate. Except for LMHW, all fractions showed antitumor activity against sarcoma 180 cells in ICR mice. The activity was the strongest in the LELFD fraction. Antitumor glucan (grifolan LE) was purified from LELFD by diethylaminoethyl-Sephadex A-25 chromatography and ethanol precipitation. Grifolan LE gave M_r of more than 5×10^6 (8 M urea/0.2 N NaOH), [α]_D -6°--9° (c=0.1%; 0.3 N NaOH), and showed metachromasy coupled with Congo red. By carbon-13 nuclear magnetic resonance spectra and methylationanalyses, grifolan LE was found to be a branched β-1,3-glucan containing a branch at C-6 of every three main chain glucosyl units.
- 社団法人日本薬学会の論文
- 1986-04-25
著者
-
大野 尚仁
東京薬科大学薬学部免疫学教室
-
大野 尚仁
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
-
宿前 利郎
Tokyo University of Pharmacy and Life Science (formerly Tokyo College of Pharmacy)
-
宿前 利郎
東京薬科大学
-
安達 禎之
Tokyo College of Pharmacy
-
安達 禎之
東京薬科大学免疫学
-
佐藤 吉朗
Nippon Beet Sugar Mfg. Co. Ltd.
-
大野 尚仁
東京薬科大学免疫学
-
大野 尚仁
帝京大学 医学部微生物学講座
-
及川 昭蔵
Nippon Beet Sugar Mfg. Co. Ltd.
-
鈴木 巌
Tokyo College of Pharmacy
-
鈴木 巖
Tokyo College of Pharmacy
-
Ohno N
Lab. Immunopharmacolgy Of Microbial Products Tokyo University Of Pharmacy And Life Science School Of
関連論文
- 薬学部新入学生の早期体験実習におけるKJ法を用いたスモールグループディスカッションの有用性
- CAWS血管炎惹起の分子メカニズム
- Inactivation of (1→3)-β-D-Glucan in Mice
- Modulation of the Antitumor Effect and Tissue Distribution of Highly Branched (1→3)-β-D-Glucan, SSG, by Carrageenan
- Relationship between the Tissue Distribution and Antitumor Activity of Highly Branched (1→3)-β-D-Glucan, SSG
- Structure-Activity Relationship of (1→3)-β-D-Glucans in the Induction of Cytokine Production from Macrophages, in Vitro
- Comparison of the Immunopharmacological Activities of Triple and Single-Helical Schizophyllan in Mice
- D-11 漢方薬熱水抽出過程での成分間相互作用への遊離アミノ酸ならびに単糖の関与
- Resistance of Highly Branched (1→3)-β-D-Glucans to Formolysis
- Structural Analysis of Alkaline-Soluble Polysaccharide, P-1,from the Kernels of Prunus mume SIEB. et ZUCC.
- Comparison of the Blood Clearance of Triple- and Single-Helical Schizophyllan in Mice
- Enhancement of LPS Triggered TNF-α (Tumor Necrosis Factor-α) Production by (1→3)-β-D-Glucans in Mice
- Enhancement of Cytokine Production by Macrophages Stimulated with (1→3)-β-D-Glucan, Grifolan (GRN), Isolated from Grifola frondosa
- Preparation and Antigen Specificity of an Anti-(1→3)-β-D-glucan Antibody
- Analysis of Cytokine mRNAs Induced by the Administration of a Highly Branched (1→3)-β-D-Glucan, OL-2
- Changes in Immune Mediators in Mouse Lung Produced by Administration of Soluble (1→3)-β-D-Glucan
- Biological Activity and Structural Characterization of Alkaline-Soluble Polysaccharides from the Kernels of Prunus mume SIEB. et ZACC.
- Expression of Interleukin 1 Family mRNAs by a Highly Branched (1→3)-β-D-Glucan, OL-2
- Immunopharmacological Characterization of a Highly Branched Fungal (1→3)-β-D-Glucan, OL-2,Isolated from Omphalia lapidescens
- SY1-4 免疫疾患に関わる真菌多糖と自然免疫受容体(真菌と免疫・アレルギー,シンポジウム1,医真菌学のコペルニクス的転回を目指して)
- P-071 シクロフォスファミド投与白血球減少症モデルマウスにおけるCandida由来PAMPsへの応答性の検討(免疫・アレルギー,ポスター討論,一般演題,医真菌学のコペルニクス的転回を目指して)
- P-072 真菌症患者血清における抗β-グルカン抗体のβ1,3/β1,6構造に対する反応性の比較(免疫・アレルギー,ポスター討論,一般演題,医真菌学のコペルニクス的転回を目指して)
- Characterization of Mitogenic Substances in the Hot Water Extracts of Bupleuri Radix
- 血管炎発症に関わる活性化好中球MPO分子の蛍光ナノ粒子による検出
- 01P2-006 DBA/2系マウスに惹起されるCAWS血管炎の心機能異常を伴う致死的病態モデルとしての有用性に関する検討(薬物療法(基礎と臨床),医療薬学の扉は開かれた)
- 免疫異常による腎臓血管傷害のイメージング
- P-153 ヒト抗β-グルカン抗体価の検討(6.服薬指導(入院・外来)2,医療薬学の未来へ翔(はばた)く-薬剤師の薬剤業務・教育・研究への能動的関わり-)
- 真菌細胞壁の構造と機能--受容体との関連も含めて (特集 糖尿病と真菌症--臨床免疫と分子生物学的アプローチ) -- (真菌に関する基礎的事項)
- ベアード・パーカー寒天培地とウサギ血しょうフィブリノーゲン寒天培地のJIS化について
- CAWSによって惹起される致死的血管炎モデル--CAWS惹起冠状動脈炎 (第1土曜特集 血管炎の基礎と臨床) -- (基礎)
- Serum Components Induce β-D-Glucan-Inhibitable Uptake of Zymosan Particles by Murine Peritoneal Macrophages
- Physicochemical Characteristics and Antitumor Activities of a Highly Branched Fungal (1→3)-β-D-Glucan, OL-2,Isolated from Omphalia lapidescens
- Structure and Antitumor Activity of the Less-Branched Derivatives of an Alkali-Soluble Glucan Isolated from Omphalia lapidescens. (Studies on Fungal Polysaccharide. XXXVIII)
- Inhibition of Expeirmental Pulmonary Metastasis of Lewis Lung Carcinoma by Orally Administered β-Glucan in Mice
- Isolation of Mitogenic Substance from Sclerotia of Sclerotinia sclerotiorum IFO 9395 Extracted with Phosphate Buffer
- Effect of Intraperitoneally Administered β-1,3-Glucan, SSG, Obtained from Sclerotinia sclerotiorum IFO 9395 on the Functions of Murine Alveolar Macrophages
- Heat Induced Generation of the Mitogenic Substance(s) Responding to Murine Splenocytes Obtained from Sclerotia of Sclerotinia sclerotiorum IFO 9395
- Macrophage Activation in Vitro by Chemically Cross-Linked (1→3)-β-D-Glucans
- Change of Biological Activities of (1→3)-β-D-Glucan from Grifola frondosa upon Molecular Weight Reduction by Heat Treatment
- Separation of the Hot Water Extracts of Sclerotia of Sclerotinia sclerotiorum IFO 9395 into Mitogenic and Antitumor-Active Subfractions
- Physicochemical Properties and Antitumor Activities of Chemically Modified Derivatives of Antitumor Glucan "Grifolan LE" from Grifola frondosa
- Antitumor and Immunomodulating Activities of a β-Glucan Obtained from Liquid-Cultured Grifola frondosa
- Conformation of Grifolan in the Fruit Body of Grifola frondosa Assessed by Carbon-13 Cross Polarization-Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy(Biological)
- Effect of Grifolan on the Ascites Form of Sarcoma 180(Biological)
- Conformational Changes of the Two Different Conformers of Grifolan in Sodium Hydroxide, Urea or Dimethylsulfoxide Solution(Biological)
- Effect of Activation or Blockade of the Phagocytic System on the Antitumor Activity of Grifolan
- Fractionation of Acidic Antitumor β-Glucan of Grifola frondosa by Anion-Exchange Chromatography Using Urea Solutions of Low and High Ionic Strengths
- Two Different Conformations of Antitumor Glucans Obtained form Grifola frondosa
- Effect of Glucans on the Antitumor Activity of Grifolan
- Characterization of the Antitumor Clucan Obtained from Liquid-Cultured Grifola frondosa
- Structure-Function Relationship of Antitumor β-1,3-Glucan Obtained from Matted Mycelium of Cultured Grifola frondosa
- Purification and Structural Characterization of an Antitumor β-1,3-Glucan Isolated from Hot Water Extract of the Fruit Body of Cultured Grifola frondosa
- Structural Characterization and Antitumor Activity of the Extracts from Matted Mycelium of Cultured Grifola frondosa
- Carbon-13 Nuclear Magnetic Resonance Spectral Analysis of the Fruit Bodies of Grifola frondosa
- Neutral and Acidic Antitumor Polysaccharides Extracted from Cultured Fruit Bodies of Grifola frondosa
- Antitumor Activity and Structural Characterization of Glucans Extracted from Cultured Fruit Bodies of Grifola frondosa
- CAWS血管炎への Bruton's tyrosine kinase の関与に関する検討
- 肺胞マクロファージの真菌細胞壁成分によるNF-κB活性化ならびにサイトカイン発現誘導の検討
- ヒト抗β-グルカン抗体のクラス別力価と反応性
- 遺伝的素因の異なるマウスを用いたCAWS血管炎の検討
- 肺胞マクロファージの真菌細胞壁成分によるNF-κB活性化ならびにサイトカイン発現誘導の検討
- 真菌細胞壁多糖に対するマクロファージの応答性
- 真菌多糖の免疫系による認識とその活性化作用
- 可溶化βグルカンの経気道曝露による気道炎症
- 可溶化βグルカンの経気道曝露による気道炎症
- 10 可溶化βグルカンの経気道曝露による気道炎症 : 第2報
- β-グルカンの構造と免疫賦活機構の解析 (特集 β-グルカンの機能性研究最新情報)
- Candida albicans培養液中の可溶性多糖画分(CAWS)によってDBA/2系マウスに惹起される血管炎の病理組織学的検討と致死毒性の解析
- P-434 血管病変解析と治療薬創生のためのCAWS誘発血管炎の応用(1.薬物療法(基礎と臨床)5,医療薬学の未来へ翔(はばた)く-薬剤師の薬剤業務・教育・研究への能動的関わり-)
- マウス脾細胞におけるbeta-glucan応答性遺伝子の網羅的解析
- 可溶化βグルカンの経気道暴露によるサイトカイン発現と気道炎症
- βグルカンの生体防御系修飾作用
- Chemical Characterization of a Fungal B-Cell Mitogen Obtained from the Fruit Body of Peziza vesiculosa
- Purification, Antitumor Activity, and Structural Characterization of β-1,3-Glucan from Peziza vesiculosa
- Antitumor Activity and Structural Characterization of Polysaccharide Fractions Extracted with Cold Alkali from a Fungus, Peziza vesiculosa
- Carbon-13 Nuclear Magnetic resonance Spectroscopy of Some Edible Mushrooms
- Conformations of Fungal β-D-Glucans in the Fruit Body of Edible Fungi Assessed by Gross Polarization-Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance Spectroscopy
- Physicochemical Properties and Antitumor Activities of Carboxymethylated Dericatives of Glucan From Sclerotinia sclerotiorum
- Examination of the Active Center of a (1→3)-β-D-Glucanase Preparation, Zymolyase
- Antitumor Activity and Structural Characterization of a (1→3)-β-D-Glucan Extracted with Cold Alkali from Sclerotia of Sclerotinia sclerotiorum IFO 9395
- Physicochemical Properties and Immunomodulating Activities of Hot Water Extract from Sclerotia of Sclerotinia sclerotiorum IFO 9395(Biological)
- Structure and Antitumor Activity of a β-1,3-Glucan Isolated from the Culture Filtrate of Sclerotinia sclerotiorum IFO 9395
- Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Various Yeasts as Aqueous Suspensions
- Generation of Cytotoxic Material to Murine Spleen Cells by Periodate Oxidation and Borohydride Reduction of Pectic Substances
- 肺炎球菌細胞壁中に存在する, リゾチーム消化抵抗性因子
- 血管炎を誘導する真菌糖蛋白質の免疫活性化 (血管炎研究がめざすあらたな展開) -- (血管炎発症機構の解析研究)
- マウス脾細胞における beta-glucan 応答性遺伝子の網羅的解析
- P-063 無脊椎動物β-グルカン認識タンパク質の結合活性におけるグルカン構造特異性の解析(一般演題(ポスター発表),基礎から臨床へ、臨床から基礎への提案)
- P-068(O2-2-5) 補体C5欠損マウスを用いたCAWS血管炎の検討(カンジダの基礎と臨床(2),一般演題(ポスター発表),基礎から臨床へ、臨床から基礎への提案)
- P-058(O2-1-3) ヒト白血球におけるβ-グルカン応答性因子の検討(カンジダの基礎と臨床(1),一般演題(ポスター発表),基礎から臨床へ、臨床から基礎への提案)
- P-056(O2-1-1) Candida albicans可溶性多糖画分CAWS投与マウス肝臓における遺伝子発現の網羅的解析(カンジダの基礎と臨床(1),一般演題(ポスター発表),基礎から臨床へ、臨床から基礎への提案)
- S4-1 真菌感染防御における好中球由来の活性酸素の役割(真菌と感染防御,基礎・臨床シンポジウム4,基礎から臨床へ、臨床から基礎への提案)
- アレルギー・炎症に関わる真菌多糖と自然免疫受容体 (特集 真菌とアレルギー・炎症)
- P-099 病原性真菌Candida albicans由来多糖CAWSの静脈内投与により惹起される血管炎の解析(一般演題(ポスター発表),生態と進化から考える医真菌学)
- P-098 CAWS血管炎のA/Jマウスを用いた解析(一般演題(ポスター発表),生態と進化から考える医真菌学)
- P-097 自然免疫系βグルカン結合タンパクのグルカン構造特異性(一般演題(ポスター発表),生態と進化から考える医真菌学)
- P-088(SS6-4) カンジダマンナンによる血管炎モデルにおける構造要求性について(一般演題(ポスター発表),生態と進化から考える医真菌学)
- P-087 カンジタ感染患者における抗β-グルカン抗体価のアイソタイプ別の検討(一般演題(ポスター発表),生態と進化から考える医真菌学)
- P-085 Candida albicans由来のβ-glucan 画分によるヒト好中球のERKおよびp38 MAPK燐酸化への影響(一般演題(ポスター発表),生態と進化から考える医真菌学)
- 好中球機能異常による感染防御能の低下と炎症の誘発