Production of Aflatoxins and Aflatoxicols by Aspergillus flavus and Aspergillus parasiticus and Metabolism of Aflatoxin B_1 by Aflatoxin-non-producing Aspergillus flavus

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The production of aflatoxin (AF) groups (B_1,B_2,G_1,G_2,M_1,M_2,P_1 and Q_1) and aflatoxicol (AFL) groups (A and B) by AF-producing Aspergillus flavus and A. parasiticus isolated from various foods, and the metabolism of AF B_1 to AF M_1,AF P_1,AF Q_1,AFL-A and AFL-B by AF-non-producing A. flavus also isolated from various foods, were studied. A simple and rapid method for the analysis of the 10 kinds of toxins was developed. Toxins produced in culture were extracted with chloroform, separated into AF and AFL fractions by a silica gel cartridge column, then assayed by high performance liquid chromatography (HPLC). AF M_1,AF M_2,AFL-A and AFL-B were detected in addition to AF B_1 and AF B_2 in nearly all of the cultures inoculated with AF-producing A. flavus. The same groups were detected in addition to AF B_1,AF B_2,AF G_1 and AF G_2 in the cultures inoculated with A. parasiticus. The time courses of AF M_1 and AF M_2 production were almost the same as those of AF B_1,AF B_2,AF G_1 and AF G_2. AFLs tended to appear after AFs had accumulated in the cultures. AF P_1 and AF Q_1 were not detected at all. AF B_1 added to the cultures was metabolized by all strains of non-AF-producing A. flavus. AFL-A and AFL-B were the common metabolites in all of the cultures. However, no strains which converted AF B_1 to AF M_1,AF P_1 and AF Q_1 were observed.
公益社団法人日本薬学会の論文
1991-04-30