Astragalus mongholicusの培養に関する研究(第1報) : 種子,幼植物,培養細胞のアミノ酸組成について

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The seeds, young plantlets and cells from the cultures of Astragalus mongholicus were investigated for their amino acid compositions. Canavanine was contained in large amounts in the seeds and young plantlets, in a small amount in the primary callus culture, and not at all in the callus tissues subcultured for two years. On the contrary, the γ-amino butyric acid (GABA) concentration in the callus was higher than those in the seeds and young plantlet. It was found that the ratio of the free amino acid content to the total amino acid content of the primary callus was higher than the corresponding ratio of the callus tissues subcultured for two years. The changes in the GABA concentration observed during the cell growth was different from the corresponding changes in the concentrations of other protein amino acids. It was observed that the cells released amino acids into the medium when they were in the stationary phases.
1991-12-20