水中微量汚染物質のマスとしての生物評価法の確立 : 細胞毒性, 変異原性, 発がんプロモーター活性

概要

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In order to establish the method of bio-assay for the safety of drinking water, promotion activity and cell toxicity of water micropollutants were studied by use of the morphological change and cell viability of HL-60 cells which were cultured with the substances recovered from raw and chlorinated waters by the adsorption with XAD-2 and activated carbon, and the results were compared with those of Ames Salmonella mutagenicity test. Among 51 fractions obtained from raw and chlorinated waters, no fraction induced differentiation of HL-60 cells to macrophage which is typically observed by the action of 12-O-tetradecanoyl-phorbol-13-acetate (TPA), suggesting no occurrence of promotor in water tested. Some fractions decreased the cell viability of HL-60,indicating the occurrence of cell toxic substances in water. Ames Salmonella mutagenicity test indicated the occurrence of mutagens in some fractions, but no correlation was observed between the results of HL-60 cell viability and mutagenic activity. These facts suggest the importance of bio-assay with culture cell systems.
社団法人日本薬学会の論文
1989-08-31