清酒釀造とビタミンB群の關係 (第2報) : 麹菌の生産する清酒酵母發育促進物質とビタミンB群特にパントテン酸との關係

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T. INOUE and A. TAKAOKA have observed the occurence of an unknown substance in the mycelia of Asp. oryzae, which stimulates the growth of Sacch. sake in a medium of high alcohol concentration.In this paper the authors have reported on the nature of the growth stimulant and its relationship to B-vitamins.1) Sacch. sake was unable to grow in a vitamin-deficient medium of relatively high alcohol concentration, but it began to grow by addition of PA and the combined use of PA, biotin, inositol and thiamine was found to promote the growth more rapidly, even though the effect was somewhat smaller than that of koji extract or the extract of the mycelia of Asp. oryzae.2) The content of free vitamins in koji extract was as follows(The amounts in 100 ml.). PA 92.5γ; inositol 415.0γ; biotin 103.0mγ; thiamine 3.2γ. The natural materials containg the B-viatmins, such as yeast autolyzate, yeast extract, rice bran extract and malt extract, also showed the same activity.3)After removal of PA from koji extract by adsorption on an anion exchanger, the effluent had no growth-stimulating effect. When PA was added to the effluent, the original growth-stimulating action was almost recovered.4) The active substance for Sacch. sake in koji extract was coincident in its position on the paper Chromatogram with that for Sacch. carlsbergensis 4228. Its locus (Rf value; 0.32 with wet n-butanol; 0.80 with n-butanol-acetic acid-water) was different from that of free pantothenic acid (Rf; 06. with wet butanol).The Rf value was analogous to that of Ca-pantothenate (Rf; 0.32 with wet butanol, 0.80 with butanol-acetic acid-water.)Perhaps the active substance might be Mg-pantothenate (Rf; 0.32 with wet butanol, 0.80 with butanol-acetic acid-water).
公益社団法人日本生物工学会の論文
1955-02-15

清酒釀造とビタミンB群の關係 (第2報) : 麹菌の生産する清酒酵母發育促進物質とビタミンB群特にパントテン酸との關係

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