清酒酵母の代謝に関する研究 : (第6報)α-フェネチルアルコールによる呼吸欠損株の誘発

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This paper deals with the induction of respiration-deficient mutant (RD mutant) in Sacch. sake by α-phenethyl alcohol (α-PEA).Yeast cells were cultivated with 0.15% α-PEA for 24 hours, diluted with sterilized water, spread on a nutrient agar plate and cutured for 2-3 days. The RD mutant was diagnosed by the colony size and the TTC (triphenyltetrazolium chloride) overlay method.About 60% of the total colonies formed on the plate were "petites" and not stained with TTC. Although the cells separated from a petite colony grew very slowly on glucose, they were not able to grow on glyceriol as a substrate. Moreover, Qo_2 of such cells was not recognized at all even after recultivation in a glucose medium free of α-PEA. Therefore, these petite colonies were considered to be RD mutant and named α-PEA mutants by the authors.Some properties of the α-PEA mutant, such as enzymatic activities and the absorption spectrum of cell suspension, were analogous to those of the mutant which is induced by acriflavin and is known as a cytoplasmic RD mutant.As mentioned in the preceding paper, the recovery of respiratory activity was observed when the α-PEA-treated cells were reincubated in an α-PEA free medium under aerobic conditions. This phenomenon is considered to be due to the concomitant presence of the cells (ca. 40% of total cells), in which the respiratory system, especially cytochrome c oxidase was reversibly inhibited by α-PEA.It has remained to be elucidated the relationship between the mechanism of the petite mutant induction and that of the reversible inhibition of the synthesis of respiratory enzymes, both of which were caused by α-PEA.
社団法人日本生物工学会の論文
1971-04-25

清酒酵母の代謝に関する研究 : (第6報)α-フェネチルアルコールによる呼吸欠損株の誘発

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