清酒酵母の代謝に関する研究 : (第2報)エタノールおよび他のアルコール類を添加して好気培養した菌体の呼吸能および醗酵能について

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This paper deals with effects of ethanol and α- and β-phenethyl alcohols on the metabolic properties of Sacch. sake grown aerobically in media containing these alcohols.From the results of manometric studies using glucose as a substrate, it was found that the Qco_2 value of cells grown in a medium containing ethanol (referred to as "ethanol-cells") was higher than that of cells grown in an ethanol-free medium of the same age. On the contrary, the Qo_2 value was lower in the former than the latter. Although the Qco_2 and Qo_2 values of both types of cells depended on their age, their R. Q. values (Qco_2/Qo_2) were almost constant : The R. Q. values of "ethanol-cells" ranged from 4.0 to 5.0,and those of the cells grown in the ethanol-free medium were about 2.8. The Q^<N_2>_<co_2> value of "ethanol-cells" was also higher, indicating that the increase of Qco_2 in "ethanol-cells" was due to enhancement of alcoholic fermentation ability. Moreover, when pyruvate was used as a substrate, the Qo_2 value of "ethanol-cells" was lower than that of the cells grown in the ethanol-free medium.In spite of a higher Qco_2 value of "ethanol-cells", no difference was observed between the pyruvate decarboxylase activities of both types of cells, whereas alcohol dehydrogenase activity of "ethanol-cells" was larger than that of the cells from the ethanol-free medium. From the results of experiments on the effect of coexistence of ethanol and glucose, it was observed that an increase of the R. Q. value was obtained only when cells were incubated under aeration in the presence of both ethanol and glucose.Among alcohols other than ethanol, α- and β-phenethyl alcohols exhibited a marked enhancing effect on fermentation activity of the yeast when added to culture media (Qo_2 decreased significantly and Qco_2 increased markedly). Especially the Qo_2 value of cells grown aerobically with α-phenethyl alcohol was reduced to almost zero.
社団法人日本生物工学会の論文
1970-07-25

清酒酵母の代謝に関する研究 : (第2報)エタノールおよび他のアルコール類を添加して好気培養した菌体の呼吸能および醗酵能について

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