清酒酵母の生理的研究 : (第5報) 乳酸含有培地に嫌気培養した清酒酵母の凝集性機構の研究 : (その1) 沈降試験法による検討

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In a previous paper a remarkable coagulation of the cells of Saccharomyces sake was observed when cultured anaerobically in the presence of exogenous lactic acid. This paper describes sedumentation experiments made to examine whether the coagulation is caused by substantial change in the cells or whether it resulted from circumstancial factors, for example, changes of pH, viscosity or specific gravity of the culture medium.The cells harvested from a lactic acid-containing medium and a lactic acid-free medium, respectively, were washed with distilled water and suspended in : 1,destilled water adjusted with MacIlvaine buffer to various pH; 2,distilled water adjusted with lactic acid and NaOH to various pH; 3. original culture medium, respectively.The sedimentation percentage value (S.P. value) and the settling velocity, V (cm/sec)of the yeast cells were measured in the various suspension media described above according to the method of Nielsen (Compt. rend. trav lab. Carlsberg., 22,61 (1937))and that of Aiba et al. (Proc. of the Symposium of Institute of Applied Mycrobiology, Univ. of Tokyo, 3,37 (1963)). In these experiments the cells cultured anaerobically in the presence of lactic acid gave significantly larger S.P. value and V than the cells harvested from lactiv acid-free medium irrespective of the suspension media so far tested.Hence, it has been concluded that the most predominant factor affecting sedimentation is the lactic acid in the culture medium. The acid causes some substantial changes in the yeast cells under anaerobic conditions. The inositol-deficient cells obtained from the inositol-free medium showed almost the same S.P. value as the cells grown anaerobically in the lactic acid-medium.These results are consistent with the present authors' observation that the presence of lactic acid resulted in a marked decreased of the bound-inositol content in the cells.
社団法人日本生物工学会の論文
1965-12-25

清酒酵母の生理的研究 : (第5報) 乳酸含有培地に嫌気培養した清酒酵母の凝集性機構の研究 : (その1) 沈降試験法による検討

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