清酒酵母の代謝に関する研究 : (第5報)α-フェネチルアルコール添加好気培養酵母の呼吸適応

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In a previous paper of this series, it was reported that the cells of Sacch. sake grown in a sythetic medium containing 0.15% α-phenethyl alcohol (hereinafter referred to as "α-PEA grown cells") lacked cytochrome c oxdase and possessed no respiratory activity.This paper deals with the recovery of respiratory activity in the "α-PEA grown cells" under various conditions.When the "α-PEA grown cells" were inoculated into an α-PEA free medium and shaked on a rotary shaker, the oxygen uptake (Qo_2) was recovered while shaking. This recovery of Qo_2 was influenced by the inoculum size of the "α-PEA grown cells" and the glucose concentration in the α-PEA free medium. With the increase of Qo_2,the activity of cytochrome c oxidase was also developed. Both cycloheximide and chloramphenicol inhibited the development of cytchrome c oxidase. This fact indicates that cytochrome c oxidase is synthesized de novo during the adaptation and the synthesis is dependent on both ribosomal and mitochondrial protein sythesizing systems.Altough these phenomena resemble the respiratory adaptation of anaerobic yeast, the recovery rate of Qo_2 was slower and the maximum value was lower than those in the respiratory adaptation of the cells grown statically on 10% glucose. This fact suggests that a part of α-PEA grown cells are respiration-deficient mutants.Among various nitrogen-containing compounds added to the medium for such adaptation tests, L-α-alanine exhibited a remarkably stimulatory effect ot the Qo_2 recovery rate of "α-PEA grown cells", but didn't influence the maximum value of Qo_2.
社団法人日本生物工学会の論文
1971-02-25

清酒酵母の代謝に関する研究 : (第5報)α-フェネチルアルコール添加好気培養酵母の呼吸適応

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