清酒酵母の生理的研究 : (第3報)清酒酵母の呼吸系補酵素およびB群ビタミン含量におよぼす乳酸の影響

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In a previous paper the occurence of the coagulation and the increase of the ratio of dead cells were observed when Saccharomyces sake was cultured statically in a synthetic medium containing lactic acid. This phenomenon is apparently analogous to the so-called "Unbalanced growth" of inositol-requiring yeast caused by the deficiency of the vitamin. In this case the amount of bound-inositol in the yeast cells was significantly lowered as the concentration of lactic acid increased.This paper deals with the marked reduction caused by the acid in the NAD, CoA and cytochrome c contents of the yeast cells. The decrease of these coenzymes was similarly observed by Ridgeway and Douglas in the case of the inositol-deficient cells exhibiting "Unbalanced growth".The decrease of several B-vitamins, such as thiamine, riboflaving and pantothenic acid, was also ascertained in the cells grown in the presence of lactic acid. Furthermore lactic acid stimulated disappearance of inositol exogenously added during the cultivation of Sacch. sake.From these results it is supposed that lactic acid blocks the transformation of inositol to its bound-form, e.g. phospholipid inositol, and causes the destruction of the structure of cytoplasma granules and phospholipid-enzyme protein complex participating in the respiratory system.
社団法人日本生物工学会の論文
1964-10-25