石油炭化水素醗酵とビタミン : (第5報)炭化水素培地におけるMycobacterium smegmatisのカロチノイド生産 : (1)培養条件の検討

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During the course of the studies on the hydrocarbon utilization and vitamin B_<12> production by several kinds of bacteria, we found out that some bacteria, such as Mycobacterium smegmatis, Nocardia lutea, and N. corallina, grew well on n-alkanes and made up a clump of cells occluding hydrocarbon. In these cases, these bacteria produced orange to orange-red pigments easily extractable with n-hexane or n-hexane-acetone mixture, which were identified as mixtures of carotenoids.In this paper, we dealt with the effects of medium constituents, aeration, and addition of some organic compounds on the carotenoid production by M. smegmatis. This bacterium could grow on n-alkanes of a medium chain-length (C_<11-19>, but not on those with shorter chains under the cultural condition used. Among pure n-alkanes, n-hexadecane was most suitable for bacterial growth and carotenoid production, but "hydrocarbon mixture 2" (rich in n-undecane) was also suitable for carotenoid production. Addition of surface detergent, Tween 80,and amino acids, such as glutamic acid, histidine, and serine stimulated the carotenoid production by M. smegmatis. Under the cultural condition tested, the maximal carotenoid production was about 800 μg. per liter of medium.
社団法人日本生物工学会の論文
1968-06-25