清酒酵母の代謝に関する研究 : (第9報) α-フェネチルアルコールの作用機構

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In a previous paper of this series, it was mentioned that α-phenethyl alcohol (α-PEA) inhibited the growth and biosynthesis of respirtory enzymes of Sacch. sake, and these phenomena would be attributed to a dual acton of the drug on two different sites of the yeast cells. In this paper, the effect of α-PEA on the respiratory adaptation of anaerobically grown cells was studied in order to obtain further information about the sites susceptible to the drug.α-PEA completely inhibited the development.of Qo_2 at a concentration higher than 0.3%. With the same at a concentration lower than 0.3%, the development of Qo_2 was also inhibited, but a complete inhibition was attained only when a high concentration of glucose, i.e. 5%, was used as a carbon source.α-PEA, like chloramphenicol (CAP), did not inhibit the induction of cytochrome c, which is known to be synthesized by the cytoplasmic ribosomal system.Moreover, as in the case of CAP, a "delayed adaptation" was observed when further adaptation was carried out in the presence of α-PEA (0.4%) after aeration with cycloheximide for 3 hr. Besides, the growth of cells was more strikingly inhibited by α-PEA under aerobic conditions than under anaerobic conditions.These facts strongly suggest that α-PEA inhibits specifically the mitochondrial protein synthesizing system. The drug induced respiration-deficient mutant and inhibited the incorporation of adenine-8-^< : 14>C into the DNA and RNA fractions, especially into the DNA fraction, at a high concentration sufficient to interfere the aerobic adaptaiton. In this case, it appears probable that α-PEA blocks the process of DNA synthesis in mitochondria.On the other hand, when yeast growht was inhibited by a low concentration of α-PEA, the total amount of adenine-8-^<14>C incoorporated into cells was decreased, but the distribution of radioactivity in cellular components was not affected. As mentioned in our previous paper, the same phenomena were observed with the incorporation of (U)-^<14>C-aspartic acid into cells. These facts would indicated that α-PEA acts on the yeast cell membrane system, independent of its action on the mitochondrial system, to inhibit the yeast growth by hindering the uptake of exogenous nutrients into the cells.
社団法人日本生物工学会の論文
1971-05-25

清酒酵母の代謝に関する研究 : (第9報) α-フェネチルアルコールの作用機構

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