清酒火落菌増殖に及ぼす脂肪酸およびその関連物質の作用(第3報) : 火落菌増殖に及ぼすオレイン酸, ビオチンおよびその拮抗体の相互関係

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Several investigators reported that biotin stimulated the growth of many strains of Hiochi-bacteria. On the other hand, WILLIAMS and FIEGER have found that the oleic acid can be used as substitute for biotin in the growth of lactobacilli.This paper deals with the interactions of oleic acid, biotin and its antagonists on the growth of Hiochi-bacteria. The results obtained are as follows : 1) In the semisythetic medium containing Sake, biotin slightly exerterd the growth-stimulating effect on all strains of Hiochi-bacteria, while in the synthetic medium three strains of the bacteria required biotin essentially for the growth. But, the addition of Tween 80 was more effective than that of biotin in both of the synthetic and semisynthetic media.2) In the synthetic medium supplied with biotin-antagonist, no growth of Hiochi-bacteria was observewd, but this inhibitory action was nullified by the addition of Tween 80.These observations suggest that the practical use of biotin-antagonist as an antiseptic of Sake is not effective, since phosphatides and fatty acids are contained in Sake.
1958-09-15