Aspergillus niger U_<20> 2-5の生産する糖化アミラーゼについて : (第4報) 糖化アミラーゼの2,3の性質

概要

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With highly purified preparation (cf. preceding report) of saccharifying amylase produced by Aspergillus niger U_<20> 2-5,the hydrolysis characteristics and amino acid composition of the enzyme were investigated.Without cooperation of α-amylase, the maximum degree of starch hydrolysis was 65% when soluble starch was used as substrate. The enzyme was capable of hydrolyzing glucosidic linkages of α-1,4 α-1,6,α-1,3 and α-1,2. Thus, maltose, isomaltose, panose, nigerose and kojibiose could be hydrolyzed, But α-1,1 linkage (α, α-trehalose) was not attacked.Amino acids produced by the hydrolysis of the enzyme protein was analyzed by two-dimensional paper chromatography, and eighteen L-amino acids were detected.
社団法人日本生物工学会の論文
1965-07-25