黒カビ糖化アミラーゼ生産におけるpreferential synthesisの生理学的解明とその多段連続培養への応用 : (第3報)preferential synthesisの要因, とくに糖化アミラーゼのmessenger RNAの安定性について

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As has already been reported, the process of glucamylase production by an adenine-requiring strain of Aspergillus niger (U20-2-5) belongs to the type of nongrowth- associated product formation. Investigations have been undertaken on the principal causes by which glucamylase production lags remarkably behind cell growth. Firstly it is deduced from the results of inhibition tests and tracer experiments with fully induced mycelia that the observed process type is really due to the preferential synthesis of the enzyme. This eliminates the possibility that the process type observed might be due to the activation of proenzyme or delayed secretion of the enzyme. Inhibition of de novo synthesis of m-RNA by the action of actinomycin D does not cause the prompt inhibition of glucamylase synthesis ; de novo synthesis of the enzyme is allowed to continue for more than 8hr in the absence of RNA synthesis. ^14C-amino acids are found incorporated in glucamylase protein synthesized during that period, indicating that the m-RNA specific for glucamylase synthesis is extraordinary stable. Based on the above results together with those already reported, main causes of preferential glucamylase synthesis are presumed as follows : (1) anomalous longevity of the m-RNA specific for glucamylase synthesis ; (2) catabolite (or anabolite) repression which is more effective as specific growth rate is higher ; (3) synthesis of the specific m-RNA lasting for a considerable period in the stationary phase of growth, as suggest in the preceding report.
社団法人日本生物工学会の論文
1966-05-25

黒カビ糖化アミラーゼ生産におけるpreferential synthesisの生理学的解明とその多段連続培養への応用 : (第3報)preferential synthesisの要因, とくに糖化アミラーゼのmessenger RNAの安定性について

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