Eremothecium ashbyiiによるリボフラビン生産における増殖負相関の要因 : (第一報)外的生産阻害因子とその制御

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The time course of riboflavin production by Eremothecium ashbyii exhibited typical features of nongrowth-associated product formation. The production was accelerated when fars and oils of vegetable and animal origin were used as carbon-energy sources. Among these, some glycerides were found to be very effective, while little acceleration of riboflavin production was observed in the culture when fatty acids and glycerol were added simultaneously.When glucose (more than 20g/l) and oil were both added initially, or after the initial phase of culture, riboflavin production was inhibited. But when glucose was fed little by little, semi-continuously, riboflavin accumulation reached as high a level as that of a culture with oil as the carbon-energy source.Glucose inhibition was observed whether or not the growth was restricted by the shortage of inositol, one of the essential growth factors for the strain employed. Other than the above glucose inhibition, a type of growth-associated inhibition was observed when the growth rate became higher due to the addition of increasing amounts of inositol.On the other hand, it was verified that the riboflavin-producing activity of cells decays remarkably when the cells are incubated in inositol-deficient conditions for a long time. Therefore, the semi-continuous addition of inositol at a very low rate was effective for the accumulation of riboflavin. A method was deduced from the above results to improve the productivity : this method consisted in a gradual (semi-continuous) and simultaneous feeding of the above two growth substrates (glucose and inositol).It was proved that the glucose inhibition, and the growth-associated inhibition of riboflavin production was minimized by the above method, while the riboflavin-producing system was protected from decay.
社団法人日本生物工学会の論文

Eremothecium ashbyiiによるリボフラビン生産における増殖負相関の要因 : (第一報)外的生産阻害因子とその制御

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