微生物酵素生産の動力学的研究 : (IV) Aspergillus nigerによる酸性プロテアーゼ生産の動力学的研究への生理学的基礎

概要

論文の詳細を見る
Employing an adenine-requiring strain (U20-2-5) of Aspergillus niger, physiological studies were conducted on acid protease production. The optimum pH for acid protease reaction was ca. 2.8. In culture filtrate at a pH range between 3.2 and 4.8,the enzyme was shown to be very stable : the activity was maintained for 75 hr at 30℃ without appreciable loss. From the results obtained so far, the acid protease activity measured is regarded to be of a single enzyme. Practically little activity of acid protease emerged in cultures grown with inorganic nitrogen compounds as sole source of nitrogen. Peptone and proteins were highly effective in inducing the enzyme. The time course of the enzyme production manifrested a distinct feature of the process type of nongrowth-associated product formation, i.e. the enzyme producing activity emerged when growth was ceased due to the exhaustion of adenine-group growth substances. Observations on the interruption of the enzyme production by inhibitors such as 5-fluorotyptophan and by the removal of oxygen, and the pattern of induction-de-induction in replacement cultures led to the conclusion that the measured increase in the enzyme activity in culture filtrate stands for the de novo synthesis of the enzyme protein. The effect of peptone in inducing the enzyme was much more remarkable when added to a culture in growing phase than in nongrowing phase. Casein hydrolyzate prepared by acid protease digestion or by trypsin digestion was also effective in inducing the encyme. Among simple chemial compounds tested for inducing action, adipic and glutaric acids were highly effective. Most of amino acids tested and some dipeptides were effective, but to much less extents than was peptone.
社団法人日本生物工学会の論文
1968-09-25

微生物酵素生産の動力学的研究 : (IV) Aspergillus nigerによる酸性プロテアーゼ生産の動力学的研究への生理学的基礎

スポンサーリンク

概要

著者

関連論文

スポンサーリンク