微生物酵素生産の動力学的研究 : (第7報) クロカビ酸性プロテアーゼの誘導の動力学

概要

論文の詳細を見る
With peptone and adipic acid as inducers, was studied the kinetics of acid protease induction in Aspergillus niger U20-2-5,an adenine-requiring strain. In adenine-limited nongrowing cultures added with peptone a latent phase lasted for about 10 hr during which time there was but slight synthesis of acid protease. The similar pattern of induction was observed with casamino acid. Among components of peptone fractionated by Sephadex, a fraction covering the molecular weight range between 1,000 and 3,000 was the most effective inducer. In remarkable contrast to the above, no time lag was observed in the induction with adipic acid as the inducer. The nature of induction lag observed with peptone was investigated, and it was revealed that mycelia could take up peptone in a short time in amounts sufficient for the active induction of acid protease after being replaced in an inducer-free medium. Therefore, the rate of induction was not limited by the rate of peptone uptake. Experiments with actinomycin D and ^<14>C-uracil added to the induction cultures at various times of incubation gave the results indicating that the time lag corresponded to the length of latent phase prior to the accumulation of the long-life mRNA specific for acid protease. It was postulated from the results that adipic acid of a substance formed rapidly therefrom might react as the inducer, while peptone would become effecive after being converted slowly into an active inducer.Deinduction experiments indicated that the longevity of acid protease-forming system was hardly affected by growth commenced on addition of adenine. But in the course of induction, added adenine caused repression closely correlated with growth.
社団法人日本生物工学会の論文
1970-09-25

微生物酵素生産の動力学的研究 : (第7報) クロカビ酸性プロテアーゼの誘導の動力学

スポンサーリンク

概要

著者

関連論文

スポンサーリンク