アラバン分解酵素に関する研究 : (IX) Aspergillus nigerによるアラバナーゼ生産の条件と経過について

概要

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Aspergillus niger strain No. 5195 produced arabanase in medium containing araban or L-arabinose. The time course of the enzyme production showed the feature of the process type of nongrowth-associated product formation. It was shown by replacement culture experiments with araban as inducer that L-arabinose added at higher concentration suppressed the enzyme production presumably because glucose instead of L-arabinose was accumulated within the cells in that condition whereas the intracellular level of L-arabinose was very low. It was verified that glucose added to the culture caused remarkable catabolite repression of arabanase formation, therefore L-arabinose per se might not cause such repression; it was rather effective as inducer when added at a low concentration. Such a repression-induction relationship was discussed in relation to the process type of arabanase formation. L-Arabitol was also active in inducing arabanase, but D-arabinose and α-phenyl-L-arabinofuranoside were not. Conditions of submerged culture for arabanase production, including the varieties of nitrogen and carbon sources and the oxygen absorption rate coefficient (Kd), were also investigated. The optimal Kd was shown to lie in the range of 4〜8×10^<-6> [mol O_2ml・min・atm].
社団法人日本生物工学会の論文
1968-09-25