ペクチン分解酵素に関する研究 : (第24報)Botrytis cinereaによるペクチン分解酵素の生産および植物組織の崩壊

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Pectic enzymes production using Botrytis cinerea and some relationships between pectic enzymes and the plant tissue macerating activities were dealt with.Botrytis cinerea was submerged cultured in a 30l jar or a 500l fermenter at 25℃. The time courses of endo-polygalacturonase (endo-PG), exo-polygalacturonase (exo-PG) and polymethylgalacturonase (PMG) productions showed good paralleling with the growth of the mold but a delayed production of pectinesterase (PE) was observed compared to its growth, suggesting that the production of PE was repressed by the hydrolytic products of pectin in the medium (Fig. 2 and 3). Analytical results, as for the relation of apparent age to the productiveness of each enzyme of the mold, indicated that PGs and PMG might be formed by the younger my celium (age 0-8 hr) and PE by the matured one (age 6-26 hr).Macerating activities on potato at pH 3.0 and on bark of Ganpi at pH 4.0 appeared to be proportional to PGs and PMG activities throughout the cultural period, while the activity of potato maceration at pH 5.0 was observed to coincide with PE activity.When cultured in a jar fermenter under a constant agitation rate (400 rpm), a seed rate of 10 to 20 ml per liter and an aeration rate of 0.75 to 1.0 liter per liter per minute were found to be adequate to give high activities of PGs, PMG and macerating activity on potato at pH 3.0 in the culture broth (Table 2).The loosening of fibrous tissue of bark and softning of vegetable foodstuffs occured when these plant tissues were immersed in a dialysed culture solution (Table 3).
社団法人日本生物工学会の論文
1967-04-25

ペクチン分解酵素に関する研究 : (第24報)Botrytis cinereaによるペクチン分解酵素の生産および植物組織の崩壊

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