スタフィロマイシン生産誘導因子の分離精製

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Extraction and purification of inducing material produced in staphylomycin fermentation. YANAGIMOTO, M., Y. YAMADA and G. TERUI (Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-kami, Suita-shi, Osaka 565) Hokkokogaku 57 : 6-14. 1979. In cultures of Streptomyces virginiae, inducing material (IM) was formed just prior to the initiation of staphylomycin production. IM had the characteristic property of inducing staphylomycin production after a short lag time when it was added to the cultrue broth at the lag period. Extraction and purfication of IM was carried out from the culture broth. Analytical data of partially purified IM, acetylated IM and IM-trimethylsilyl ether indicated a molecular formula of C_<12>H_<22>O_4 for IM. IM has a γ- or δ-lactone group and two hydroxy groups. IM was estimated in the form of carboxylic acid to have two hydroxy groups at γ- and δ- positions, another hydroxy group as a hydroxybutyl group, and two methyl groups. γ-Undecalactone, γ-nonalactone and γ-octalactone are effective in inducing staphylomycin production at the concentration of 2 μg/ml, 20 μg/ml, 100 μg/ml, respectively. IM is effective at a concentration of less than 0.02 μg/ml. Under normal culture conditions, 32 units/ml of IM was produced, which was equivalent to less than 0.6 μg/ml.
社団法人日本生物工学会の論文
1979-01-25