リグニンモデル化合物3-(2-methoxy-4-formylphenoxy)-1,2-propanediolの細菌による分解

概要

論文の詳細を見る
Lignin is widely distributed in plants. Its structure has not been established yet due to its complexity, but it involves a phenylpropane structure with ether bonds. Rod fungi are prevalent in the decomposition of lignin, but are not applicable to the treatment of pulp wastes because of the slow decomposition. Much effort has been taken to find good microbes which decompose ligin and most isolated strains mainly or only utilized carbohydrates contaminating in lignin. As the determination of lignin is not easy, a model compound with the basic structure of lignin was selected to find lignin-decomposing microorganisms. As a model compound, 3-(2-methoxy-4-formylphenoxy)-1,2-propanediol (MFP) was synthesized from vanilline and a-glyceromonochlorohydrin. Mixed culture M-9 was obtained from sludge. It utilized MFP as a single carbon source, and degraded 0.1% MFP completely in 3 days. Three bacterial strains, P, Y, and W, were isolated from the M-9,but could not grow on MFP as a single carbon source either singly or mixed. Strains Y and W were identified to belong to Flavobacterium ferungium and F. ringense, respectively, but the strain P was similar to F. letescense and seemed to be a new species. By the addition of glycerol to a basal medium, strains Y and W degraded 40% and 60% of MFP (0.1%) in 60 hr, respectively, but the strain P only oxidized the aldehyde group to the carboxyl, which was isolated and confirmed to be 3-(2-methoxy-4-carboxyphenoxy)-1,2-propanediol by the comparison of chemically oxidized MFP. The dialysis culture by using cellophane membrane showed that the inability for the 3 strains to grow on MFP was not due to the inhibitor formation during MFP metabolism nor due to the lack of some essential nutrients. Also these strains could not grow on the culture filtrate of M-9. The existence of some other microbes than these 3 strains was suggested in the decomposition of MFP.
社団法人日本生物工学会の論文
1976-09-25

リグニンモデル化合物3-(2-methoxy-4-formylphenoxy)-1,2-propanediolの細菌による分解

スポンサーリンク

概要

著者

関連論文

スポンサーリンク