グルタミン酸醗酵の酵素レベルでの解析 : 第1報 α-ケトグルタール酸脱水素酵素とその経時変化について

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1. Changes of metablic activities and quotients were investigated first in cultures of Brevibavterium sp., a typical biotin-requiring glutamic acid (GA) former, and the results suggested a achange in α-ketoglutaric acid-oxidizing activity due to biotin-deficiency.2. In view of the known metabolic routes in connection with the above results, the behavior of α-ketoglutaric acid dehydrogenase (αKGDH) activity was considered to be of a crucial importance in elucidating the change from the growth phase to the phase of flutamic acid formaiton. The results obtained in this respect were as follows : a) The highest recoveries of α-KGDH activity from Brevivacterium cells were obtained with acetone-dried preparations. The optimum pH for the reaction was 6.7,and the K_mvalue for α-ketoglutarate was 9.52×10^<-4>M, which was much higher than the values of αKGDH from other organism.b) In the phase of GA produciton, the αKGDH activity per cell was lowered to about 60 % of the value obtained with actively growing boitin-sufficient cells.c) At the culture age of 24 hr, i.e. in the late nongrowing phase, the αKGDH activity of cells was as low as about 40 % of the activety observed in 24-hr-old cells grown with an excessive supply of biotin throughout the culture.
社団法人日本生物工学会の論文
1971-05-25