糸状菌の生産するセルラーゼに関する研究 : (第1報) ペニシリウムセルラーゼのパルプ繊維に対する作用について

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As previously reported from this laboratory, the cellulase system of Penicillium variabile consists of three componens, C_1 C_x and cellobiase, and attacks native cellulose to produce glucose. In this paper, was dealt with the mode of degradation of wood pulp using partly purified preparation of the enzyme system. Refined wood pulp powder (100 mesh in particle size) used in our experiments consisited of cellulose moleccule with D.P. of ca. 1000; the weight ratio of crystalline and amorphous regions was 3 : 7,In the initial phase of enzymic attack, the D.P. was quickly decressed presumably as a result of hydrolysis occurred in the amorphous regions arranged alternately with the crystalline regions (Fig. 6), and the residual substrate became less hygroscopic because of the amount of more hygroscopic (amorphous) region was decreased (Fig. 5 (a)). Dissolution analysis with caustic soda solution showed that the content of crystalline region was increased in this initial phase (Fig. 4(a)). In the subsequent phase, outer part of cystalline region got loose becoming more amorphous; thus the residual substrate became more hygroscopic and the amorphous regions thus formed anew were then attacked and dissolved, while no remarkable change of D.P. in the residual substrate was observed. The above sequence of structural change in the wood pulp is illustrated diagrammatically in Fig. 7.
社団法人日本生物工学会の論文
1965-05-25