液内培養によるRhizopus属の糖化型アミラーゼ生産 : (第3報)紫外線照射によるRhizopus javanicus sp.3菌の改良

概要

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As reported previously, a large amount of saccharogenic amylase was produced by submerged culture of Rhizopus javanicus sp. 3,the amount being equal to that of black koji mold. In this study, for the purpose of increasing saccharogenic amylase activity, improvement of this strain was tried. By the ultraviolet ray irradiation at 6cm distance to sporangiospore of this strain (1×10^5 spores in 1ml of 0.1M, pH 6.0 phosphate buffer solution) for 1.25 and 1.75 minutes, 693 survivals were obtained. From these survivals 441 strains were cultured on koji agar slant at 30℃ for 7 days. As the first selection 44 strains were selected from 441 strains by macroscopic morphological observation, a secondary 16 strains were selected by measuring Lintner value of culture filtrate, and finally 2 strains were selected by measuring saccharogenic amyrase activity of culture filtrate. Saccharogenic amylase activity of culture filtrate produced by these two strains was increased about 20% and 15% respectively as compared with that of the parent strain. It is of interest that sporangium formation of these two strains was inferior to that of the parent strain.
社団法人日本生物工学会の論文
1963-12-15