麹酸醗酵に関する研究 : (第7報) グルコースからの生合成機構

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The conversion of glucose to kojic acid (5-hydroxy-2-hydroxymethyl-γ-pyrone) by Aspergillus oryzae No. 69-20 U in a submerged culture was investigated. Using 1- ^<14>C-, 6- ^<14>C-, 3- ^3H-, and 5- ^3H-glucose as substrates, the distribution of radioactivity in kojic acid obtained by fermentation of ^<14>C-glucose and the incorporation of ^3H into kojic acid were examined. Mycelia pre-cultured for 48 hr, were suspended in media containign labeled glucose and cultured for 48 hr. The kojic acid obtained was isolated from the culture filtrate by precipitation with copper acetate. ^<14>C-Kojic acid was converted to 2-ethyl-6-methyl kojic acid by ethyl iodate and dimethyl sulfate. This derivative of kojic acid was hydrolysed by a Ba(OH)_2 solution and fractionated on a Hyflo Super-cel column to give ethoxyacetone (2,3,4-C), methoxyacetone (4,5,6-C), formic acid (1-C), ethoxyacetic acid(2,3-C), and methoxyacetic acid (5,6-C).The following results were obtained : 1) Between 80 and 90 % of the total radioactivity of the isolated kojic acid was found in the carbon atoms corresponding o those labeled in the glucose 2) When 3- ^3H-glucose and 5- ^3H-glucose were used as substrates, the incorporation of ^3H into kojic acid was slight, 3.5 and 1.35 %, respectively.These results indicate that the major pathway of kojic acid formation in a submerged culture is a direct conversion of glucose without any splitting of the carbon chain.
社団法人日本生物工学会の論文
1971-10-25

麹酸醗酵に関する研究 : (第7報) グルコースからの生合成機構

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