石油化学製品の醗酵に関する研究 : (第3報)土壌中より分離したPseudomonas属細菌によるプロテアーゼの生産について

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A taxonomic study was performed on a strain of soil bacteria which could grow and produce in a medium containing petrochemicals as a source of carbon.For this reason, the strain is considered to belong to Pseudomonas aeruginosa as described in Bergey's Manual (7th ed.).Although this strain assimilates various alcohols (except for methanol), glycols (except ethylene glycol), and n-paraffinic hydrocarbons (C<13>-C<17>), the substrates for production of the protease were considerably limited.The effective substrates for production of the protease were ethanol, n-propanol, n-hendecanol, n-dodecanol, 1,2-propanediol, and 2,3-butanediol.The protease activity of the culture broth, an inorganic salt medium containing 2 vol % ethanol after cultivation at 30℃ for 5 days, was ca. 50 units.The optimal reaction pH, the stability range of pH, and the optimal reaction temperature of this protease were 8.5,7.0〜9.0,and 50℃, respectively.Furthermore, the protease tolerated treatment at 50℃, at pH 8.5 for 30 min.
社団法人日本生物工学会の論文
1972-11-25