Micrococcus flavusの生成するホルムアルデヒド酸化酵素 : 微生物によるホルムアルデヒドの分解.(第2報)

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A formaldehyde-oxidizing enzyme was partially purified form the cell extract of Micrococcus flavus by treatment with streptomycin sulfate, precipitation with ammonium sulfate and chromatography on hydroxylapatite column.The optimum pH and temperature of the enzyme were 6.0 and 30℃. The enzyme was stable below 30℃ and in the range of pH 6.0 to 6.5. This enzyme acted specifically on formaldehyde. THe action of this enzyme on formaldehyde was conducted without hydrogen acceptors, such as NAD, NADP, methylene blue and 2,6-dichlorophenol indophenol. In addition, oxygen in the reaction system was consumed during the oxidation of formaldehyde to formic acid by this enzyme. On the basis of these facts it is probable that the enzyme belongs to the aldehyde oxidases (EC 1.2.3.1.) which had not been found in microbes.
社団法人日本生物工学会の論文
1974-11-25