液内培養によるRhizopus属の糖化型アミラーゼ生産 : 第1報菌株の選択および糖化型アミラーゼの性質

概要

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In the alcoholic fermentation industry, the submerged culture of Rhizopus for production of saccharogenic amylase is being carried out, using the amylo process or the combined koji-amylo process. However, because it is difficult for a culture filtrate containing a large amount of saccharogenic amylase to be produced by these culture methods described above, solid culture is generally being used for production of saccharogenic amylase of Rhizopus.For the purpose of producting a culture filtrate containign a large amout of saccharogenic amylase, saccharogenic amylase producing power of several strains of Rhizopus by submerged culture was examied and one of a distinguished strain, Rhizopus javanicus sp. 3,was selected.It was found that, from the saccharogenic amylase producing point of view, a submerged culture was superior to a solid culture for this strain.The amylolytic enzyme system in the culture filtrate of this strain was chiefly occupied by saccharogenic amylase and only to a small degree by dextrinogenic amylase and transglucosidase.It was found that the optimum pH of this saccharogenic amylase was within the range of from 4.5 to 5.0,stable pH was within the range of from 4.0 to 8.0,and the optimum temperature was about 50℃ in low concentration of starch.
社団法人日本生物工学会の論文
1963-10-15