麹酸醗酵に関する研究 : (第6報)工業化について

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Kojic acid production on an industrial scale and its recovery by concentrating the cultural filtrate were examined. The maximum kojic acid accumulation (32g/l) in a 30l jar fermentor (working volume 15l) was obtained at 1 vvm (aeration rate) and 400 rpm (agitation speed). The Kd value by the sulfite-oxidation method was 11.2×10<-6> g-mol O_2/min., atm., ml under the same conditions. A test on the oxygen transfer coefficient (Kd) was carried out. on a larger scale with a 300l fermentor (working volume 100l).The relation between the agitation speed of impeller and the Kd value is shown in Fig. 4,where the aeration rate was 1 vvm. There was a linear relation when plotted on the logarithimic scale. Accordingly, the agitation speed necessary to obtain the Kd value of 11.2×10<-6> g-mol O_2/min., atm., ml in the 300l fermentor is 240 rpm. Then, kojic acid fermentaiton was carried out under the same conditions as above. The cultural course (Fig. 5) was similar to that of the 30l jar fermentor and the amount of kojic acid was 29.4g/l Therefore, the scale-up with a 300l fermentor based on the Kd value seemed to be successful.Kojic acid production in 1500l tank (woring volume 700l) was carried out at 1 vvm and 220 rpm and 29g/l of kojic acid was accumulated (Fig. 6). Then, the recovery of kojic acid by concentrating the above culture medium was examinated. After filtrating the culture medium, the filtrate was concentrated under a reduced pressure. The 1st crystalline kojic acid (11.67 kg) was obtained when the filtrate (720l) was concentrated to 171l. The precipitated kojic acid was centrifuged off and the supernatant was again concentrated to 39.4l. Then, the 2nd crystalline kojic acid (5.87 kg) was obtained. After the above supernatant was concentrated to 7.2l, the 3rd crystals (2.18 kg) were obtained by standing the solution at 0℃.The purity of the 1st, 2nd and 3rd crystals of kojic acid was 90%, 80% and 34% respectivery. The total yield of kojic acid from the cultural filtrate was 86.9%.
社団法人日本生物工学会の論文
1971-04-25

麹酸醗酵に関する研究 : (第6報)工業化について

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