メラノイジン系物質の微生物の代謝生産物におよぼす影響について

概要

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It has been confirmed that over-heated sterilization of a medium lowers the yield of microbial metabolic products and this low yield partly results from browing materials present in the medium.Assuming that these browning materials were melanoidine substances (the products of Mailard reaction), we discussed the effects of melanoidine substances on α-amylase and s-amylase production by Asp. awamori var. fumeus, and α-amylase production by Bac. subtilis under submerged culture conditions.Melanoidine substances were synthesized by interaction between sugars and amino acids, mostly glucose and glycine. The reaction was allowed to proceed one hour at 130℃.When added to the media in Table 1 and 9,melanoidine substances increased the production of amylolytic enzymes. From the results of Table 7 and 8,we recognized that the action of melanoidine substances was more than that of a simple nutrient.
1966-08-25