連続培養法によるClostridium butyricum Miyairiの胞子生産

概要

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Production of spores of Clostridium butyricum Miyairi 642 was investigated in two-stage continuous cultivation. The medium was composed of corn starch, amino acids mixture and calcium carbonate. In the first vessel, the optimal dilution rate was 0.50-0.60hr^<-1> for vegetative cell formation, and the cell count reached 1.0-1.1×10^9 ml^<-1>.This count was greater than that in batch culture (specific growth rate=0.85 hr^<-1>, maximal cell count=3.7×10^8 ml^<-1>). In the second vessel, better spore formation was observed when dilution rate was less than 0.075 hr^<-1>. The maximal spore count was 4.0×10^8 ml<-1>. The spore formation of C. butyricum Miyairi 642 in the continuous culture did not depend on dilution rate of the first vessel, but on that of the second vessel.
社団法人日本生物工学会の論文
1980-11-25