菌体における酸素の拡散を伴う消費について : 拡散を考慮した場合とMichaelis-Mentenの式の比較

概要

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In analysing the rate of respiration of microbes, it seems to be of significance to consider the mechanism of oxygen diffusion within cells together with the enxymatic reaction of zero order.In this attempt to take the two mechanisms into account simultaneously, the correlations pertaining to oxygen consumption were made.In the value of the C_<se>/C_<lc> was assumed to be in a range extending from 0.85 to 0.95 and N/N_m from 0.65-0.8 to 0-0.15,the term which should correspond to the constant in the Michaelis-Menten's equation has been shown to remain inchanged irrespective of the dissolved oxygen concentration of in the medium. This analysis proceeded to determine the critical levels of oxygen in the case of S. cerevisiae and of rat liver cells. The agreement between the determination of the critical levels of oxygen from these calculations and the experimental data published by other investigators was favorable.It was also found that the liquid film coefficient around the cells was of the same order of magnitude as that found around air bubbles in aerated systems.The results of this study are considered to be comprehensive enough to verify the reasonableness of the theory outlined in a previous report.
社団法人日本生物工学会の論文
1963-03-15