Heparin Inhibits the Progression Phase of Subcultured Endothelial Cell Proliferation in Rat Aorta.
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概要
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The anti-proliferative effects of heparin on subcultured endothelial cells (EC) of rat aorta were investigated to determine whether heparin inhibits the competence phase or the progression phase in the cell cycle using the starting time and the rate of proliferation. Fetal bovine serum (FBS)-stimulated EC proliferation increased to 1.5-times in cell number, but decreased to 0.5-times in <SUP>3</SUP>H-thymidine incorporation, compared with the proliferation of rat primary cultured smooth muscle cells (SMC). The FBS-effects on EC proliferation were attributed to the progression phase rather than the competence phase. Heparin (1, 10 or 100 μg/ml) significantly inhibited the proliferation of FBS (5%)-stimulated EC, and the extent of inhibition was the same in both cell number and <SUP>3</SUP>H-thymidine incorporation. In the <SUP>3</SUP>H-thymidine incorporation every 3 hr, heparin reduced the rate of incorporation into G<SUB>0</SUB>-arrested EC, but did not affect the starting time of DNA synthesis. When the index of competence phase, starting time, was plotted against that of progression phase, rate of proliferation, the inhibition of heparin was attributed to the progression phase. These results demonstrate that heparin selectively inhibits the progression phase in subcultured EC of rat aorta.
- 公益社団法人 日本薬理学会の論文
著者
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Naitoh Takeshi
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceut
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Kimura Masayasu
Deparment Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
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Kimura Ikuko
Deparment Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Kobayashi Shinjiro
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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Nagaura Takeshi
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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Kobayashi Shinjiro
Department of Applied Chemistry, Faculty of Engineering, Kyushu University
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