グルタミン酸の醗酵生産とその応用に関する研究 : (第1報) 通気攪拌量のグルタミン酸生産に対する影響について

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The effects of aeration on the growth of test organisms and on their abilities to produce glutamic acid were studied on the scales of shake flask and jar fermenter using strain No. 761-8 and No. 425-40 which belonged to the genus Brevibacterium.Oxygen absorption coefficient Kd was used as an indicator of aeration efficiency. The cell weight of test organism increased with the increase of Kd up to a value of 4.3×10^<-6>g-moles O_2/ml.min.atm. in shaking culture. Since the glutamic acid formation of the test organisms was most active in the early period of stationary growth phase, it was desirable to control the degrees of agitation and aeration, in order to get a suitable amount (about 8 mg/ml in shaking culture) of the test orgaism by that period. When suitable amounts of organisms were present in the fermentation medium, the glutamic acid formation was maximum at Kd value of about 4.3×10^<-6>. Investigations on oxygen supply, oxygen demand of organism and dissolved oxygen are necessary for reasonable agitation and aeration in a large scale fermenter. The autors examined the changes of the respiration rate of organism and amounts of dissolved oxygen during the glutamic acid fermentation. There was not so simple relationship between the production and respiratory activity in the glutamic acid fermentation as the direct correlation between the aeration efficienty and the glutamic acid formation. The amount of dissolved oxygen in fermentation broth was measured by polarographic technique using stationary platnium electrode inserted into fermenter.
社団法人日本生物工学会の論文
1960-03-15

グルタミン酸の醗酵生産とその応用に関する研究 : (第1報) 通気攪拌量のグルタミン酸生産に対する影響について

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