Bacillus polymyxa 1変株の生成するBacterial Polysaccharideの研究(第8報) : タンク培養に依る生産(其の2)

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Although the authors reported in the preceding paper on the poroduction of the polysaccharide by using a small fermenter, they have now studied the industrial production by submerged culture and economically possible purification methods. The results were as follows : 1) It was recognized that the production of the polysaccharide was considerably influenced by aeration and agitation during fermentation, and in the case of 1001 scale fermentation, aeration more than 1001 per min at 165r.p.m. of agitation resulted in the maximum production of the polysaccharide with sufficient bacterial growth. Generally, the fermentation was completed in a week, when initial lactose conc. was 5〜10%.2) As a result of examining some precipitants, the authors have preferred i-propanol for the isolation of the polysaccharide from the broth, since it was precipitated from the aqueous solution by adding 55〜65% (v/v) of i-poropanol.In the process of purification, protein was completely removed by autoclaving crude polysaccharide solution at pH 5.8 for 10 min without considerable decomposition of the polysaccharide. Furthermore, paritially depolymerized products of desired grade could be obtained by treating the polysaccharide with dil. HCl(pH1〜2) at 70〜80℃, and their yields were usually 8〜15% on the basis os the lactose utilized.
社団法人日本生物工学会の論文
1958-02-15