B. polymyxa 1変株の生成する細菌多糖類ラムノガラクタンの研究 : (第13報)部分解合物の調製と分子量の測定

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The authors studied the partial depolymerization of the native rhamnogalactan and the fractionation with some water-miscible precipitants to prepare the materials of desired molecular size. And, they determined weight-average molecular weights of variously depolymerized fractions. The results obtained are as follows : 1) The native rhamnogalactan was readily depolymerized to desired degrees without considerable decomposition by dilute acid, e.g., H_2SO_4,HCl (pH, 1.5〜2.0) or oxalic acid, at 70〜80℃. But no hydrolysis was brought about by the action of Takadiastase.2) Rhamnogalactan was precipitated by successive addition of 65〜80% ethanol (in the presence of small amoutn of alkali) or 55〜70% i-propanol to its aqueous solution, but it was not precipitated with methanol (90% conc.).A better fractionation was carrid out by successive addition of ethanol (until 42.8% conc.) or i-propanol (until 50% conc.) after dissolving the depolymerized material in methanol. By such procedures, the fractions ([η] : 0.07〜0.3)-differing in molecular size were prepared.3) Weight-average molecular weights of the different materials ([η] : 0.136〜1.805) so obtained were determined by the light-scattering method, and the following experimental equation was obtained for fractions having molecular weights less than 150,000 : [η]-3.084×10^<-5>・M^<0.85>_W
社団法人日本生物工学会の論文
1958-08-15